Plasmids and viral vectors are traditionally used in CRISPR genome editing to express the required proteins. However, there is a risk to use DNA instead of mRNA: Double stranded DNA breaks catalyze insertion of DNA at the cut site. At some substantial frequency, the protein expression vectors can integrate, which can lead to continuous expression of the nuclease or a previously silent sequence.

Now mRNA is becoming a powerful tool in genome editing. With no risk of insertional mutagenesis, mRNA are used to transiently express the required proteins. Wild-type Cas9 mRNA is a stabilized non-immunogenic messenger RNA (mRNA) that has been designed to produce high expression level of wild-type Cas9 protein. It creates a double stranded break at a target site delineated by RNA guide sequences.

This mRNA is produced by in vitro transcription, stabilized at the 5’ end by modified nucleotides capping and contain a poly(A) tail at the 3’ end. mRNACas9 is optimized to yield improved stability and performance and to lower the cellular innate immunity response.

mRNA transfection provides several advantages :

• No need of nuclear uptake - protein expression directly in cytoplasm

• Faster protein expression than DNA transfection

• No genomic integration

• Perfect for transfecting slowing or non-dividing cells

• Protein expression in a total promoter-independent manner

• Transient transfection: mRNA based expression of proteins sustains for a limited time

Size: 20 µg or 100µg

For bulk, please contact us: order@ozbiosciences.com

Storage: -80°C

mRNAs ARE SHIPPED ON DRY ICE

Dry ice fee included in shipping cost

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OZ Biosciences

OZ Biosciences成立于2003年，为全球科学界和制药业生产专用的转染、转导试剂。公司专注于DNA、RNA和蛋白质等生物分子的体外和体内应用交付技术。

自2003年以来，OZ Biosciences为全球科学界和制药行业创建，开发和生产专门用于转染和转导工具的创新分子递送系统。OZ Biosciences专注于体外和体内应用的生物分子（例如DNA，RNA和蛋白质）的输送技术。 

在过去的几年中，生命科学经历了技术革命。在基因组学，功能基因组学和蛋白质组学领域所取得的进展为更好地理解基本生物学过程和开发下一代药物创造了绝佳的机会。

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