Reporter genes are commonly used in cell biology research. 

OZ Biosciences offers reporter mRNAs that can be used as controls to study transfection and expression in mammalian cells using a variety of assays. They mimic fully processed mature mRNA.

These mRNAs are produced by in vitro transcription and stabilized at the 5" end by modified nucleotides capping (Cap1). They contain a poly(A) tail at the 3" end and are optimized to yield improved stability and performance.

They are modified with 5-methoxyuridine (5moU replaces U) to reduce innate immune responses.

We also offer unmodified mRNAs -- #MRNA9 (Tomato), #MRNA15 (GFP), #MRNA16 (F-Luc).

We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.

mRNA transfection provides several advantages :

• No need of nuclear uptake - protein expression directly in cytoplasm

• Faster protein expression than DNA transfection

• No genomic integration

• Protein expression directly related to mRNA quantity

• Perfect for transfecting slowing or non-dividing cells

• Protein expression in a total promoter-independent manner

• Transient transfection: mRNA based expression of proteins sustains for a limited time

• GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. 

• F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase protein. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
• Tomato mRNA has been designed to produce high expression level of Orange Fluorescent Protein. The detection can be done by fluorescent or confocal microscopy. The produced Tomato has an excitation maximum at 551-557 nm and emission maximum at 579-583 nm. Tomato expression level can also be monitored by fluorescence-activated cell sorter analysis (FACS).

• mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is photostable and resistant to photobleaching. 
• β-galactosidase (β-gal) mRNA, codes for a protein product of the bacterial LacZ gene. β-gal catalyzes the conversion of β-galactosides into monosaccharides. it is a common marker gene used to assess transfection efficiency. 

Size: 20 µg, 100µg and 1mg.

For bulk, please contact us: order@ozbiosciences.com

Storage: -80°C

mRNAs ARE SHIPPED ON DRY ICE

Dry ice fee included in shipping cost

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The following items are 5 moU modified mRNAs -- if you need unmodified mRNAs, please visit this page.

OZ Biosciences

OZ Biosciences成立于2003年，为全球科学界和制药业生产专用的转染、转导试剂。公司专注于DNA、RNA和蛋白质等生物分子的体外和体内应用交付技术。

自2003年以来，OZ Biosciences为全球科学界和制药行业创建，开发和生产专门用于转染和转导工具的创新分子递送系统。OZ Biosciences专注于体外和体内应用的生物分子（例如DNA，RNA和蛋白质）的输送技术。 

在过去的几年中，生命科学经历了技术革命。在基因组学，功能基因组学和蛋白质组学领域所取得的进展为更好地理解基本生物学过程和开发下一代药物创造了绝佳的机会。

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