The ROS Detection Assay kit (Green Fluorescence) is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).

Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.  

Main Features

• Measure ROS production in live cells

• Ideal for fluorescence microscopy, microplate titration and cytometry

• Compatible with adherent cells as well as suspension cells

Kit content

Tert-Butyl Hydrogen Peroxide (TBHP) 25 mM (50 µL)

Each ROS Kit provides sufficient reagents for approximately 300 measurements in microplate format and includes Tert-Butyl hydroperoxide (organic peroxide) solution provided as a positive control for ROS generation. 

Storage: Upon reception, store the DFC-DA tube (avoid multiple freeze/thawing cycles) and the 10X ROS Buffer at -20°C. Store the TBHP tube at 4°C.

Shipping Conditions: Shipped with ice pack

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OZ Biosciences

OZ Biosciences成立于2003年，为全球科学界和制药业生产专用的转染、转导试剂。公司专注于DNA、RNA和蛋白质等生物分子的体外和体内应用交付技术。

自2003年以来，OZ Biosciences为全球科学界和制药行业创建，开发和生产专门用于转染和转导工具的创新分子递送系统。OZ Biosciences专注于体外和体内应用的生物分子（例如DNA，RNA和蛋白质）的输送技术。 

在过去的几年中，生命科学经历了技术革命。在基因组学，功能基因组学和蛋白质组学领域所取得的进展为更好地理解基本生物学过程和开发下一代药物创造了绝佳的机会。

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